Biochemistry

Chem 460, 3 Credits

Spring 2010  MWF 3:00


Instructor            Dr. David A. Boyles, Room 319

PhD Synthetic Medicinal Chemistry; Minor: Natural Products Chemistry, Purdue University, 1988


Prerequisite        Chem 328, Organic Chemistry II


Text              Rodney Boyer, Concepts in Biochemistry, Third edition, Pacific Grove: Brooks/Cole Publishing Company, 2004.


Purpose            To address major topics of modern biochemistry with emphasis on (1) biomolecules, their structure, function, conformation, and self-assembly into higher order structures and systems, (2) metabolism and bioenergetics, including catabolism and anabolism of fundamental molecules, and (3) expression and transmission of genetic information. Mother nature is the greatest designer of all!


Pace of Course This is a college upper level course building on two semesters of freshman chemistry and two semesters of organic chemistry.  Study and pace yourself for a science course at this level.  University policy indicates two hours of outside study for each credit hour of class.   Some students may, however, need to allot more time than this.  


Course Structure 

You must stay up with the reading assignments and the problems at the end of each chapter.  This course is not a depth course but is a broad, one-semester introduction which covers the variety of topics found in the biochemical literature today.


Outcomes    The student who has successfully completed this course will be able to perform the following as presented in the first semester material of this course:

1.         Describe the structures and functions of organelles in the cell;

2.         Describe the structures and functions of organic molecules found in living systems;

3.         Describe the major metabolic pathways and cycles for anabolic and catabolic processes from the standpoint of the molecular level;

4.         Understand the energy changes involved in biochemical systems;

5.         Use technical terminology to discuss the integration of oxidative pathways related to energy storage and utilization;

6.        Describe the events involved in replication, transcription, and translation from the standpoint of the molecular level;

7.        Be able to think critically in terms of the material learned within the discipline.


Requirements            Attendance is mandatory.  Quizzes, homework, and/or examinations are due when assigned.  No makeup quizzes or examinations will be given outside of school policyI abide by University Policy to the letter in the case of school-sponsored absences.  Two quizzes and no more will be discarded.


Grading  Your grade is determined by points earned on exams, quizzes, and other assignments given during the semester.  

Final grades will be calculated according to the following scale:  

                        A            92-100              B-            80-81                D+          69

                        A-           90-91                C+          79                     D            60-68

                        B+           89                     C            72-78                F            0-59

                        B            82-88                C-            70-71

I curve in favor of students but only after all points are in.  No one receiving fewer than 50% of the points in the course will be passed, and I think long and hard about passing anyone with fewer than 60% of the points.


Office Hours        These will be posted early in the semester.  You do not need an appointment during office hours.  Other times only by appointment;  use the sign-up sheet by the door. At the current time my schedule looks like this. This is not a distance class.  Requests are to be registered in person.  I do not return phone calls except in the most extreme of circumstances and prefer not to honor impersonal hiding behind email  when face-to-face is far preferable. Below is the class and exam schedule subject to modification. Genetic information is often moved to the end of the semester.

Class period              Topic                                   Chapter                 Pages

         1             Introduction to biochemistry and DNA       1                        1-35

         2             Biomolecules in water, bonding                2                        36-47

         3             pH, pK, and buffers                                 2                        48-62 

         4             Amino acids                                           3                        63-74

         5             Peptides, proteins, 1° structure                3                        74-94

6                        Proteins, 2° and 3° structure                    4                        95-111

7                        Proteins, 4° structure, examples              4                        112-130

8                        EXAM I

9                        Enzymes, intro, kinetics, reactions          5                       131-145

10                   Enzymes, mechanisms, inhibition             5                      145-162

11                   Enzymes, coenzymes, regulation              6                      163-180

12                   RNA and other biocatalysts                       6                      180-195

13                   Carbohydrates, mono-, di-                         7                      196-212

14                   Carbohydrates, poly-, glycoproteins           7                      213-230

15                   Lipids, structure, function                          8                      231-245

16                   Lipids, steroids, membranes                     8,9                   245-266

17                   Membrane transport                                 9                      266-281

18                   EXAM II 

19                   DNA, RNA, structure/function I                10                      282-299                

20                   DNA, RNA, structure/function II               10                      299-314

21                   DNA replication and repair                       11                     315-336

22                   Transcription                                            11                   336-351

23                   Translation                                               12                   352-370

24                   Post-translational processing, regulation     12                  370-388

25                   Recombinant DNA and biotechnology         13                  389-416

26                   EXAM III

27                   Introduction to metabolism                        14                   417-434

28                   Bioenergetics                                           14                   434-448

29                   Carbohydrate metabolism I                        15                   449-463

30                   Carbo. metabolism II, PDHase                 15,16                464-494

31                   Citric acid cycle, NADH prod.                   16                    494-512

32                   Electron transport                                     17                   513-524

33                   Oxidative phosphorylation                          17                   524-531

34                   Photosynthesis                                         17                 531-554

35                   EXAM IV                              

36                   Lipid metabolism I                                     18                555-576

37                   Lipid metab. II, cholesterol                        18                576-591

38                   Nitrogen, amino acid metabolism              19                592-608

39                   Amino acid metabolism, urea cycle           19                608-626

40                   Integration of metabolism I                        20                627-638

41                   Integration of metabolism II, obesity          20                639-651


Freedom in Learning: Under Board of Regents and University policy student academic performance will be evaluated solely on an academic basis, not on opinions or conduct in matters unrelated to academic standards. Students are free to take reasoned exception to the data or views offered in any course of study and to reserve judgment concerning what are matters of opinion, and they are responsible for learning the content of any course of study in which they are enrolled. Students who believe that an academic evaluation reflects prejudiced or capricious consideration of student opinion or conduct which is unrelated to academic standards set forth for the class may contact the dean of the college which offers the class  to initiate a review of the evaluation.

Any alleged violations must be substantiated and documented and first discussed with the faculty member by the student as per published university grievance process. Whether malicioius or not, unsubstantiated, arbitrary, capricious, or false accusations by students are against the 9th Commandment: "Thou Shalt Not Bear False Witness Against Thy Neighbor" (Exodus 20:16). Teach thyself to speak truth and learn that what thou doest say is not always truth.

 

Students who may require technical assistance in compliance with the Americans with Disabilities Act must contact the instructor and the campus ADA coordinator, Ms. Jolie McCoy, at 394-1924, at the earliest opportunity. I will discuss these matters only in person.

Electronic Devices Policy.  Please turn off your cell phone before class starts. No use of electronic/computer media is allowed during class time in this class.